Allergen Elisa II Kit for Total Milk
- Detection of allergens in highly processed and heated foods
- High sensitivity (LOD: 0.31 ppm)
- High specificity
- Easy-to-use protocols
ELISA II Morinaga Allergen Kit – Cutting-edge ELISA technology for ultimate sensitivity in allergen testing.
These ELISA kits are sandwich enzyme immunoassays for the quantitative determination of individual proteins from allergenic ingredients in processed or unprocessed foods. These kits are designed for use by quality control personnel or other trained professionals for allergen surveillance in production or for product release controls.
Proteins in the samples are extracted, centrifuged, and filtered before the sandwich ELISA. The antigen binds to the polyclonal antibody-coated wells of the microplate module, resulting in an antigen-antibody complex. The enzyme-conjugated antibody then binds to the already bound antigen-antibody complex, forming an antibody-antigen-antibody sandwich.
The addition of enzyme substrate results in the development of color, the intensity of which can be determined by the absorbance at 450 nm. The intensity of the color developed is directly proportional to the protein concentration of the allergenic ingredients in the food.
1 x 96-well microplate (12 strips of 8 wells)
Buffers and associated enzymes
Item number:
Measurement time:
Sample preparation time: Approx. 10 min
Sample extraction time: Approx. 30 min, or overnight
ELISA analysis: Approx. 2 h
Assay sensitivity: 0.31 ppm (μg protein/g feed)
Detectable concentration range: 0.78 to 50 ppb of detectable protein
Storage: At 2-8°C (35-46°F), DO NOT FREEZE
Working temperature: 20-30 °C (all solutions must be balanced)
Extraction procedure
Short-time extraction method
Grind/chop the samples
Weigh 1.0 g of sample into a tube, add 19 mL of sample extraction solution.
Incubate the covered tube in boiling water for 10 minutes.
Chill the tube and shake for 30 seconds.
Adjust pH to 6.0-8.0.
Centrifuge and filter the supernatant if necessary.
Dilute the sample extract 20 times with diluent I.
Overnight extraction method
Grind/chop the samples
Weigh 1.0 g of sample into a tube, add 19 mL of sample extraction solution.
Attach the tube to a shaker horizontally and rock at room temperature overnight.
Adjust pH to 6.0-8.0.
Centrifuge and filter the supernatant if necessary.
Dilute the sample extract 20 times with Diluent I.
ELISA procedure
Pipette 100 μL of Standard Working Solution and Working Sample into a well of the microplate.
Incubate the microplate for 1 hour at 20-30°C (68-86°F).
Wash the wells 6 times with Wash Solution.
Dispense 100 μL of Enzyme-Conjugated Antibody (F).
Incubate the microplate for 30 min at 20-30°C (68-86°F).
Wash the wells 6 times.
Dispense 100 μL of Enzyme Substrate (G).
Incubate the reaction for 20 min at 20-30 °C (68-86 °F) in the dark.
Stop the enzymatic reaction by adding 100 μL of Stop Solution (H).
Measure Absorbance at 450 nm.
Calculate the detectable protein concentration using the standard curve.