Checklite AT100
- Highly sensitive microbial biomass assay based on ATP measuring method
- No centrifugation required
- Reduces time & labor of aseptic testing for milk, low acidic beverages etc
- Easy to use
| Verpackungsgröße: 1 Stk. |
CheckLite AT100 is a microbial biomass assay kit based on the ATP measuring method. It is especially suitable for aseptic test of low acidic beverages and retort foods after appropriate incubation. It contains thermostable firefly luciferase and an ATP eliminating reagent.
Luciferase specifically reacts with ATP and a directly proportionate amount of bioluminescence is produced. The ATP levels due to extracellular ATP of emulsive products, such as milk, cream, and products containing milk can be several orders of magnitude higher than the background ATP levels found in other food products. This high level of ATP interferes with the sensitive detection of intracellular ATP from contaminating microbes. With the CheckLite AT100, somatic cells and micelle structure are ruptured and the released ATP and any extracellular ATP are degraded to very low concentrations compared with other ATP methods, enabling the sensitive detection of bacteria in emulsive product samples.
1 x 100 Assays
Kit Composition
Luciferin-luciferase reagent HS - 2 vials deep green-labeled
Reconstitution buffer for Luciferin-luciferase reagent - 5.5 ml x 2 vials pale pink-labeled
ATP releasing reagent - 5.5 ml x 2 plastic vials light blue-labeled
ATP eliminating reagent - 2 vials reddish pink-labeled
Reconstitution buffer for ATP eliminating reagent - 5.5 ml x 2 vials yellow-labeled
Sample dilution buffer - 25 ml x 4 plastic bottles dark blue-labeled
Sample treating reagent - 5.5 ml x 2 plastic vials brown-labeled
The concentration of ATP in living cells changes rapidly. Therefore, extract ATP from cells or freeze the cells immediately after sampling. Even in the case of extraction, the inhibition or destruction of ATP-degrading enzymes may be necessary to prevent the enzymes from decreasing the concentration of the ATP.
Prepare the bioluminescence, and ATP eliminating reagent according to the manual. After preparation of the samples, reagent and samples are mixed to start the reaction.
By measuring the bioluminescence in a Luminometer, such as the recommended C-110, the level of ATP can be determined. These results can be quantified by plotting against a prepared standard curve. The number of microbial cells (Colony Forming Units, CFU) can be obtained based on the correlation between the amount of ATP and the number of CFU counted beforehand according to the traditional colony counting method.
For more details, please consult the manual.