GAM Broth is a liquid medium for anaerobic bacteria used for common culture and susceptibility test. Agar is removed from GAM Agar. As the medium contains the digested serum that has hemin, it is successfully used for cultivation of anaerobic bacteria. Hemin serves as essential growth factor along with other peptones and extracts in the media whereas Sodium chloride maintains the required osmotic equilibrium.
Scope of delivery
Specifications
| Article number |
1005422 |
| Storage conditions |
Keep dry at room temperature |
| Expiration |
Three (3) years after manufacturing |
| Form |
Powder |
Instructions
Extraction procedure
- Suspend 59.0 g of the dehydrated medium in 1,000 mL of distilled water, and heat to dissolve the medium.
- Distribute the medium into test tubes or flasks and sterilize by autoclaving at 115°C for 15 minutes.
- For cultivation of anaerobic bacteria, cool the medium immediately after the sterilization in running water without shaking or moving, inoculate the organisms at once with a sterilized capillary or a Pasteur pipette, and incubate under the anaerobic condition.
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