EC Blue 100 is a simple, quick and reliable test method for the qualitative and quantitative determination of coliforms and E.coli in water. Add water to be tested into a pre-prepared, sterile, non-fluorescent EC Blue 100 container. Then incubate the mixture at 35 ±2 °C. The medium does not require further preparation work. This means that the test can be carried out anywhere without much effort. Traditional incubating methods require about three days for the detection of coliforms or E.coli. ECBlue, on the other hand, already provides reliable test results after 24 hours. The detection is based on a colour reaction and is thus easy to interpret: if coliform bacteria are present, the water turns blue due to the cleavage of the chromogenic substrate X-Gal. In the event of faecal contamination by E.coli, these bacteria can only be conclusively detected under UV light (reaction with a fluorescent dye). Suspended matter, cloudy, yellowish or chlorinated water samples do not generally interfere with detection. EC Blue has a shelf life of up to two years at room temperature if protected from light.