Eagle’s Modified Essential Medium

Article no: 1005901
  • Has growth-promoting effect for cells such as HeLa or L and other cell lines
  • Autoclavable medium
90,00 
107,10  incl. 19% VAT 
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Delivery status: Currently not in stock

Eagle’s MEM is a powdered medium prepared according to prescription published by Harry Eagle in 1959. Up to now, such media were not autoclavable because of decomposition or deterioration of the constituents, so that sterilization was performed by filtration using a membrane filter.

This medium is specially manufactured so that constituents of the medium and growth of cells with this medium are not impaired.

Scope of delivery

  • 1 x 100 g

Specifications

Article Number 1005901
Storage conditions 2 - 5°C in a dark and dry place
Expiration One (1) years after manufacturing
Form Powder

Instructions

Extraction procedure

  • Dissolve 9.4 g of the medium in distilled water and adjust the volume to 1,000 mL
  • Sterilize by autoclaving at 121 centigrade for 15 minutes.
  • Cool the sterilized medium to room temperature and add a proper amount of sterile 10 % sodium bicarbonate solution. (pH will be 7.1 - 7.4 at 37 °C in an atmosphere of 5 % C02 in air by adding 12.5 - 22.0 mL of sodium bicarbonate solution).This mixture should be closed tightly and stored in a cool, dark place
  • At the time of use, aseptically add 0.292 g of L-glutamine (sterilized) and the desired amount of serum.

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