Eagle’s Modified Essential Medium

Extraction procedure

• Dissolve 9.4 g of the medium in distilled water and adjust the volume to 1,000 mL
• Sterilize by autoclaving at 121 centigrade for 15 minutes.
• Cool the sterilized medium to room temperature and add a proper amount of sterile 10 % sodium bicarbonate solution. (pH will be 7.1 - 7.4 at 37 °C in an atmosphere of 5 % C02 in air by adding 12.5 - 22.0 mL of sodium bicarbonate solution).This mixture should be closed tightly and stored in a cool, dark place
• At the time of use, aseptically add 0.292 g of L-glutamine (sterilized) and the desired amount of serum.