Dilution Rack- PBS for Compact Dry

Extraction procedure

• Punch two holes into the foil of each of the wells.
• After preparing the homogenized sample, drop 1 mL of the sample with a sterile pipette into the well through one of the holes. (for a ten times dilution, 10-1)
• Mix by pipetting up and down.
• Use a new pipette, entering through the other punch hole and draw up 1 mL and transfer onto a Compact Dry plate (see below).
• In order to generate further dilutions, transfer 1 mL sample to the next well (dilution 10-2) and so on. Repeat this step until you get to the dilution calculated.