Compact Dry SL

Extraction procedure

Preparation of Apparatus and Materials

• Prepared and sterilized medium made from Buffered Peptone Water (BPW) or EEM Broth Hyserve Ident No. 1000071
• Sterilized homogenize bag with filter
• Homogenizer
• Stand for homogenize bag
• Sterilized disposable pipette (1mL) or sterilized measuring pipette
• Sterilized water
• Incubator (36±1°C and 42±1°C)

Preparation of Specimen

• Solid Foodstuffs:
• Take 25g of solid specimen into the sterilized homogenizer bag. Add 225 mL of sterile Buffered Peptone Water or EEM Broth into the bag, and homogenize with stomacher for about one (1) minute.
• Water or Liquid Foodstuffs:
• Add 9 times volume of Buffered Peptone Water or EEM Broth to liquid specimen. Filtrate the liquid sample through membrane filter, and put the filter into BPW or EEM Broth.
• Wiped sample:
• Add 9 times volume of Buffered Peptone Water or EEM Broth to the whole liquid made from wiped samples.

Direction

• Prepared specimen shall be kept in the closed homogenized bag, and incubate the bag 20 - 24 hours at 35 – 37 °C in the incubator for pre-enrichment.
• Take the bag out from the incubator and rub the bag for homogenization. Use sterilized disposable pipette for sample inoculation. Drop 0.1 mL (3 drops from the 1 mL pipette) of enriched specimen on the dry sheet (approx. 1 cm far from the edge of plate) gently. This enriched culture will stay at dropped point. Diffusion of this dropped specimen shall not reach to the edge of plate.
• After the inoculation of the enriched culture, drop 1mL of sterilized water gently at the opposite point where the specimen dropped. Sterilized water will diffuse automatically and the sheet will be wet uniformly.
• Turn over the plate capped, put in an incubator and incubate 20 - 24 hours at 41 – 43 °C.