CheckLite HS Set is a kit for microbial biomass assay based on the ATP measuring method. It contains thermostable firefly luciferase and ATP degrading enzymes developed by Kikkoman Corp. The bioluminescence reagent contains firefly luciferin and luciferase. Luciferase specifically reacts with ATP and catalyzes the reaction to produce bioluminescence. This bioluminescence is directly proportional to the amount of ATP in the sample.
All living cells have ATP as their energy source. Therefore, the total cell mass can be determined by measuring the bioluminescence with the luciferase reaction, after extracting ATP from the cells using the ATP releasing reagent in this kit.
| Article Number | 1002650 |
| Test duration | 60-90 mins (including calibration curve) |
| Storage | 2-8°C, DO NOT FREEZE |
| Label | Name of component | Content | Quantity |
|---|---|---|---|
| 1 | Luciferin-luciferase reagent HS | 2 vials deep green-labeled | |
| 2 | Reconstitution buffer | ||
| 3 | ATP releasing reagent | ||
| 4 | ATP eliminating reagent | ||
| 5 | Reconstitution buffer for ATP eliminating reagent | 5,5 mL | 2 vials yellow labeled |
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