CheckLite HS Set is a kit for microbial biomass assay based on the ATP measuring method. It contains thermostable firefly luciferase and ATP degrading enzymes developed by Kikkoman Corp. The bioluminescence reagent contains firefly luciferin and luciferase. Luciferase specifically reacts with ATP and catalyzes the reaction to produce bioluminescence. This bioluminescence is directly proportional to the amount of ATP in the sample.
All living cells have ATP as their energy source. Therefore, the total cell mass can be determined by measuring the bioluminescence with the luciferase reaction, after extracting ATP from the cells using the ATP releasing reagent in this kit.
Scope of delivery
| Article Number
| Test duration
|| 60-90 mins (including calibration curve)
|| 2-8°C, DO NOT FREEZE
|| Luciferin-luciferase reagent HS
|| 2 vials deep green-labeled
|| Reconstitution buffer
|| ATP releasing reagent
|| ATP eliminating reagent
|| Reconstitution buffer for ATP eliminating reagent
|| 5,5 mL
|| 2 vials yellow labeled
- The concentration of ATP in living cells changes rapidly. Therefore, extract ATP from cells or freeze the cells immediately after sampling. Even in the case of extraction, the inhibition or destruction of ATP-degrading enzymes may be necessary to prevent the enzymes from decreasing the concentration of the ATP.
- Prepare the bioluminescence, and ATP eliminating reagent according to the manual. After preparation of the samples, reagent and samples are mixed to start the reaction.
- By measuring the bioluminescence in a Luminometer, such as the recommended C-110, the level of ATP can be determined. These results can be quantified by plotting against a prepared standard curve. The number of microbial cells (Colony Forming Units, CFU) can be obtained based on the correlation between the amount of ATP and the number of CFU counted beforehand according to the traditional colony counting method.
- For more details, please consult the manual.