CheckLite CT150 is an enzymatic presence-absence test for total coliforms based on the detection of beta-galactosidase. Recommended media contain surfactant to propagate only gram-negative bacteria. The Detection Reagent CT150 contains D-luciferin-O-b-galactopyranoside (LuGal), a substrate derivative of both b-galactosidase and luciferase. Luciferin liberated from LuGal by b-galactosidase can be detected as luminescence using luciferase. The amount of bioluminescence produced in the reaction above is in direct proportion to the amount of b-galactosidase in the cultured and permeabilized coliform bacteria.
It is a highly sensitive bioluminometric assay which allows more rapid detection of coliforms than colorimetric assays or fluorescent assays.
Scope of delivery
Specifications
| Article Number |
1002652 |
| Test duration |
5,5 – 24,5 hrs |
| Storage |
2-8°C, DO NOT FREEZE |
Kit components
| 1 |
Detection Reagent CT150 |
|
5 green-labeled vials |
| 2 |
Solvent Water for detection reagent CT150 |
|
5 pink-labeled vials |
| 3 |
Positive Control CT150 |
2 mL |
1 blue-labeled vial |
Instructions
Extraction procedure
- The test sample is coliform positive when the luminescence value of the test culture is higher than 2 times the blank value (Value 3). If it is more than 1.5 times but less than 2 times, the sample may be coliform positive. In such cases, we recommend you measure the test culture again with an extra hour of incubation, which may make the results more definitive. If it is less than 1.5 times, the test sample is coliforms negative.
- For a detailed description of the workflow, please consult the manual.
