CheckLite CT150 is an enzymatic presence-absence test for total coliforms based on the detection of beta-galactosidase. Recommended media contain surfactant to propagate only gram-negative bacteria. The Detection Reagent CT150 contains D-luciferin-O-b-galactopyranoside (LuGal), a substrate derivative of both b-galactosidase and luciferase. Luciferin liberated from LuGal by b-galactosidase can be detected as luminescence using luciferase. The amount of bioluminescence produced in the reaction above is in direct proportion to the amount of b-galactosidase in the cultured and permeabilized coliform bacteria.
It is a highly sensitive bioluminometric assay which allows more rapid detection of coliforms than colorimetric assays or fluorescent assays.
| Article Number | 1002652 |
| Test duration | 5,5 – 24,5 hrs |
| Storage | 2-8°C, DO NOT FREEZE |
| Label | Name of component | Content | Quantity |
|---|---|---|---|
| 1 | Detection Reagent CT150 | 5 green-labeled vials | |
| 2 | Solvent Water for detection reagent CT150 | 5 pink-labeled vials | |
| 3 | Positive Control CT150 | 2 mL | 1 blue-labeled vial |
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