CheckLite AT100 is a microbial biomass assay kit based on the ATP measuring method. It is especially suitable for aseptic test of low acidic beverages and retort foods after appropriate incubation. It contains thermostable firefly luciferase and an ATP eliminating reagent.
Luciferase specifically reacts with ATP and a directly proportionate amount of bioluminescence is produced. The ATP levels due to extracellular ATP of emulsive products, such as milk, cream, and products containing milk can be several orders of magnitude higher than the background ATP levels found in other food products. This high level of ATP interferes with the sensitive detection of intracellular ATP from contaminating microbes. With the CheckLite AT100, somatic cells and micelle structure are ruptured and the released ATP and any extracellular ATP are degraded to very low concentrations compared with other ATP methods, enabling the sensitive detection of bacteria in emulsive product samples.