CheckLite AT100

Article no: 1002659
  • Highly sensitive microbial biomass assay based on ATP measuring method
  • No centrifugation required
  • Reduces time & labor of aseptic testing for milk, low acidic beverages etc
  • Easy to use
498,00 
592,62  incl. 19% VAT 
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Delivery status: Currently not in stock
CheckLite AT100 is a microbial biomass assay kit based on the ATP measuring method. It is especially suitable for aseptic test of low acidic beverages and retort foods after appropriate incubation. It contains thermostable firefly luciferase and an ATP eliminating reagent.

Luciferase specifically reacts with ATP and a directly proportionate amount of bioluminescence is produced. The ATP levels due to extracellular ATP of emulsive products, such as milk, cream, and products containing milk can be several orders of magnitude higher than the background ATP levels found in other food products. This high level of ATP interferes with the sensitive detection of intracellular ATP from contaminating microbes. With the CheckLite AT100, somatic cells and micelle structure are ruptured and the released ATP and any extracellular ATP are degraded to very low concentrations compared with other ATP methods, enabling the sensitive detection of bacteria in emulsive product samples.

Scope of delivery

  • 1 x 100 Assays

Specifications

Article Number 1002659

Kit components

Label Name of component Content Quantity
1 Luciferin-luciferase reagent HS 2 vials deep green-labeled
2 Reconstitution buffer for Luciferin-luciferase reagent 5,5 mL 2 vials pale pink-labeled
3 ATP releasing reagent 5,5 mL 2 plastic vials light blue-labeled
4 ATP eliminating reagent 2 vials reddish pink-labeled
5 Reconstitution buffer for ATP eliminating reagent 5,5 mL 2 vials yellow-labeled
6 Sample dilution buffer 25 mL 4 plastic bottles dark blue-labeled
7 Sample treating reagent 5,5 mL 2 plastic vials brown-labeled

Instructions

Extraction procedure

  • The concentration of ATP in living cells changes rapidly. Therefore, extract ATP from cells or freeze the cells immediately after sampling. Even in the case of extraction, the inhibition or destruction of ATP-degrading enzymes may be necessary to prevent the enzymes from decreasing the concentration of the ATP.
  • Prepare the bioluminescence, and ATP eliminating reagent according to the manual. After preparation of the samples, reagent and samples are mixed to start the reaction.
  • By measuring the bioluminescence in a Luminometer, such as the recommended C-110, the level of ATP can be determined. These results can be quantified by plotting against a prepared standard curve. The number of microbial cells (Colony Forming Units, CFU) can be obtained based on the correlation between the amount of ATP and the number of CFU counted beforehand according to the traditional colony counting method.
  • For more details, please consult the manual.

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