These ELISA kits are sandwich enzyme immunoassays for the quantitative determination of individual proteins of allergenic ingredients in processed or unprocessed food. These kits are designed to be used by quality control personnel or other trained professionals, for allergen surveillance in the production or for product release controls.
Proteins in the samples are extracted, centrifuged and filtered prior to the sandwich ELISA. Antigen is bound to the polyclonal antibody coated wells of the microplate module resulting in an antigen-antibody complex. Subsequently, the enzyme-conjugated antibody is bound to the already bound antigen-antibody complex, forming an antibody-antigen-antibody sandwich.
Addition of enzyme substrate results in color development, and the intensity of which can be determined by the absorbance at 450 nm. The intensity of the color developed is directly proportional to the concentration of protein of allergenic ingredients in the food.
| Article Number | 1 500 007-2 |
| Sample preparation time | Approx. 10 min |
| Sample extraction time | Approx. 30 min, or overnight |
| ELISA analysis | Approx. 2 h |
| Assay Sensitivity | 0.31 ppm (μg protein /g food) |
| Detectable concentration range | 0.78 to 50 ppb detectable protein |
| Storage | At 2-8°C (35-46°F), DO NOT FREEZE |
| Working temperature | 20-30°C (All solutions should be equilibrated) |
| Label | Name of component | Content | Quantity |
|---|---|---|---|
| A | Reagent A (10X Concentrate) | 100 mL | 1 Bottle |
| B | Reagent B (10X Concentrate) | 100 mL | 1 Bottle |
| C | Reagent C (10X Concentrate) | 100 mL | 1 Bottle |
| D | Antibody-coated Microplate Module | 6X8 well modules | 2 packs |
| E | Standard (50 ng/mL extracted protein solution) | 1 mL | 2 vials |
| F | Enzyme-conjugated Antibody | 13 mL | 1 Bottle |
| G | Enzyme Substrate (TMB Solution) | 13 mL | 1 Bottle |
| H | Stop Solution (1N Sulfuric Acid) | 13 mL | 1 Bottle |
| I | Wash Buffer (20X Concentrate) | 50 mL | 1 Bottle |
| Frame for mounting the microplate module | 1 Piece | ||
| Microplate cover | 1 Piece |
Abonnieren Sie unseren Newsletter und verpassen keine Angebote mehr!
Our company is located in the most beautiful nature in Bavaria and yet in the immediate vicinity of the important logistics center of Munich. This location advantage and our flat hierarchies enable us to do something we are particularly proud of: our extremely short delivery times. Contact us!
Quality control and
Food safety: The protection
of your health through
microbiological know-how -
that's what HyServe stands for.
