These ELISA kits are sandwich enzyme immunoassays for the quantitative determination of individual proteins of allergenic ingredients in processed or unprocessed food. These kits are designed to be used by quality control personnel or other trained professionals, for allergen surveillance in the production or for product release controls.
Proteins in the samples are extracted, centrifuged and filtered prior to the sandwich ELISA. Antigen is bound to the polyclonal antibody coated wells of the microplate module resulting in an antigen-antibody complex. Subsequently, the enzyme-conjugated antibody is bound to the already bound antigen-antibody complex, forming an antibody-antigen-antibody sandwich.
Addition of enzyme substrate results in color development, and the intensity of which can be determined by the absorbance at 450 nm. The intensity of the color developed is directly proportional to the concentration of protein of allergenic ingredients in the food.